Biodiesel Machine January 16, 2017, lab of Gregory Stephanopoulos of metabolization project big ox publishs in NATURE BIOTECHNOLOGY transform yeast of solving fat Ye (Yarrowialipolytica) the literary works that raises yield of ester of fatty acid armour: Lipid Production In Yarrowia Lipolytica Is Maximized Byengineering Cytosolic Redox Metabolism. Yeast of solving fat Ye is heart thing to synthesize tristearin acid to produce redundant NADH by dextrose, what need synthesis of fatty acid of NADH translate into NADPH via transforming, make strength of production of ester of fatty acid armour amounts to 1.2 G/L/h, process yield is amounted to 0.27, relatively yeast of the project before this raises 25 £¥ , be close to NREL technology index (90 G/L, 1.3 G/L/h, 0.28 G-fatty Acids/g-lignocellulosic Sugars) .
Yeast of solving fat Ye is mode petroliferous yeast, be transformed extensively to use but second birth raw material produces oil kind fuel and chemical, but only product of a few high additional cost (be like ω-3 fatty acid, EPA) be able to commercialize promotion, and the control that the promotion of fuel of low cost biology counts raw material cost greatly, the total yield that needs to raise former expect grease and change rate.
This research learns a model to evaluate blame fat to give birth to corporeal yield character through number above all (YB) , cellular fat qualitative content (C) synthesize way yield character with fat (YL) 3 elements are qualitative to fat process yield (Y) influence, when sending low now biomass, cellular oil content is to rise ratio is crucially; When high oil content, grease must be led to the theory of dextrose is crucial factor. Accordingly the article aims to raise theory to must be led. To tristearin acid according to dextrose complex balances type, will redundant NADH is changed into fatty acid to synthesize needs NADPH, .
Be based on this, they pass NADPH of translate into of qualitative NADH of afterbirth of 4 kinds of strategy general or acetyl complementary enzymatic A, use in order to save the electron in heart thing at child synthesis. Cross expressive acetyl in setting bacterium individual plant above all complementary enzymatic A carboxyl is changed enzymatic (ACC) with 2 acyl base glycerine acyl base move is enzymatic (DGA) as individual plant of bacterium setting out (AD) . Pass in AD further (1) cross expression to turn hydrogen is enzymatic, invalid. (2) the aldehyde of former unripe glycerine that depends on NAD dehydrogenation of 3 phosphoric acid is enzymatic replace the different source version that depends on for NADP to obtain ADgapc2, , rise 29% . (3) acetone acid / careless acyl second is acerbity / the apple is acerbity (POM) the acid of different source apple that NADP conveys to depend on too in the loop is enzymatic, yield rises to 0.21. (4) expressive bowel film the phosphoric acid of bacterium of bright a string of beads turns the phosphoric acid acetyl of shuttle bacterium transfers ketone enzymatic He Keshi enzymatic introduce NOG, biomass and oil content have rise, but must lead have no apparent promotion. In addition, convey NAD kinase to also can raise child to change too rate. Combination is politic (2) with strategy (3) total yield of ester of acerbity armour of tristearin of project bacterium ADgm achieves 0.28g/g. #p#·ÖÒ³±êÌâ#e#
Finally, through filling group by group makings control ferments condition, ADgm synthesizes chroma of ester of fatty acid armour to achieve 99g/L, produce intensity 1.2 G/L/h, process yield amounts to ester of 0.27 G- fatty acid armour / G- dextrose, be close to NREL to give straw candy grease the target of set.