Biodiesel Production Line suppliers
Biology derv bases is ester of fatty acid armour (or second ester) , be wait for oil-bearing crops with soja and rapeseed, or the fatty acid in animal fat and methanol (or alcohol) the child of reaction, be a kind with petrifaction derv close, store carry uses the green with safer, good performance but second birth the sources of energy, the most promising development makes fossil fuel replace the sources of energy. The industrialized production methods of biology derv basically has chemical way at present (soda acid catalysis) with biology law (enzymatic catalysis) two kinds of main forms. Chemical law (acerbity law or alkaline law) existence production specific power consumption is big, by-product glycerine depart is difficult, product aftertreatment is complex and pollution is discharged wait for a problem. Enzymatic law production overcame chemical law catalysis completely to produce afore-mentioned a lot of defect in the process, reduced production to craft asks and produce cost, it is the development way that biology derv industrialization produces future.
Use lipase to produce biology derv to exist a major challenge: Activator lipase is water-solubility, and heart thing oil is hydrophoby, the contact that this brings about lipase and grease is inadequate, catalytic efficiency is not quite tall. Be aimed at this problem, the research group that Sun Chang Kim heads hopes the exterior structure that adopts lipase is transformed, the heart thing that enhances lipase can reach a gender, improve the catalytic efficiency of lipase then. This research is published at ACSCatal for the problem with ”Recombinant Lipase Engineered With Amphipathic AndCoiled-Coil Peptides “ . 2015, 5, 5016−5025.
The author is research target with lipase M37, try to go to the polypeptide join of two close sexes on M37 above all, inspected BR2, BR3, NKC3 the polypeptide of different length is enzymatic to M37 vivid influence, discover NKC can enhance enzymatic vigor significantly; Then the polypeptide that the author inspects different will curly helix (the CCPs) influence to enzymatic work, decided MAT has optimal effect. Curly helix polypeptide conduces to enzymatic with enzymatic the assembly between, in water oil the interface forms a structure that exceeds an element, conduce to the union of long chain fatty acid and active center.
Get through transforming recombine quantity of lipase NKC-M37-MAT element to be 164kD, in solution with 4 get together the form of body exists. Opposite at female parent, should recombine enzymatic catalytic vigor to raise 54 times, thermal stability rises significantly, tm value increased 8 ¡æ . Recombine this enzymatic the production that is used at biology derv, make production periodic shorten considerably, decrease suddenly from 30 H to 6 H. This kind of method is decorated for enzymatic surface provided new thinking and means.